122799荧光素钾盐

D-荧光素（ D-Luciferin )是荧光素酶(Luciferase）的常用底物 ，普遍应用于整个生物技术领域，尤其是体内活体成像技木。其作用机制是在ATP和荧光素酶的作用下，荧光素(底物） 能够被氧化发光。荧光素能够穿透细胞膜，可以用来监测体内细胞的活动，因为与荧光素酶的反应依赖于ATP，所以細胞活性也可以确定。

Optimized D-luciferin for in vivo Imaging

Luciferin is a chemical substance found in the cells of various bioluminescent organisms. When Luciferin is oxidized under the catalytic effects of luciferase and ATP, light is produced. Because the reaction is dependent on ATP, it allows researchers to determine the presence of energy or life. Firefly luciferin is a particularly good reporter for in vitro imaging due to properties of its emission spectra.

Luciferin can be used in a number of ways. It can be used in a variety of in vitro assays, where the production of light can be monitored with either a luminometer or a scintillation counter. It can also be used to monitor light production in vivo, and can be monitored with PerkinElmer's IVIS^® imaging platform. Because luciferin can penetrate cell membranes, it allows transformed cells to be monitored for luciferase activity.

Luciferin can also be used with PerkinElmer's IVISbrite luciferase expressing tumor cell lines and our IVISbrite lentiviral particles. There are many considerations when choosing a luciferin substrate and it is important to know that you are using the highest quality Luciferin for your experiments.

This IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate is available in a 1g unit size.

Molecular Information: C11H7 KN2 O3 S2 (MW: 318.4)