jetOPTIMUS®
| Reagent |
jetOPTIMUS® DNA Transfection Reagent |
|---|---|
| Molecule delivered |
Plasmid DNA |
| Applications |
Transient and stable gene expression from plasmid DNA transfection |
| Cell types |
Adherent cells and hard-to-transfect cells: |
| Number of transfections |
1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol |
| Storage |
Store jetOPTIMUS® at 5 °C ± 3°C. |
| Provided with |
jetOPTIMUS® Buffer
|
Superior gene expression in easy-to-hard-to-transfect cells
jetOPTIMUS® improves DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.
High gene expression using low DNA amount
jetOPTIMUS® has been developed to increase transfection efficiency into a wide range of cell types, while keeping the amount of DNA as low as possible and reducing the volume of reagent required compared to main competitors.
Excellent cell viability and morphology
Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, preserving cell viability and morphology, which allows generation of biologically relevant data from gene expression studies.
Time-saving with optimized ready-to-use protocol
jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).
